The room houses some large painted canvases including the one with St. John the Baptist and St. Benedict, attributed to Alessandro Rosi, with a central window that allows the view of the 1400 fresco with the Madonna and Child in the church of the Virgin of Ferruzza. It follows the canvas by Michele Laschi da Fiesole with the Virgin among the saints Michael the Archangel, Nicola from Tolentino and Nicola from Bari, and the one with St Luigi Gonzaga by Giovan Domenico Ferretti. A recent acquisition is the 1600s canvas with St Sebastiano by an anonymous Venetian painter. This last one comes from the Landini-Marchiani collection along with two Triumphs exhibited in the entrance hall of the museum.
In the display case there is a brass ewer of the 1500s, a silver monstrance by Francesco Loi, the processional cross from a Florentine workshop dating to the early 1700s, and the lectern donated by Caterina Ferrini in 1741 to the altar of the Conception in the church of St Salvatore.

From this room it is possible to see the upper portion of a large fresco attributed to Cenni di Francesco di Ser Cenni (late 14th-ealry 15th century).